Anti-TCR alpha + TCR beta antibody [H57-597] (ab25336)
- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-TCR alpha + TCR beta antibody [H57-597]
See all TCR alpha + TCR beta primary antibodies -
DescriptionArmenian hamster monoclonal [H57-597] to TCR alpha + TCR beta
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Host speciesArmenian hamster
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Specificityab25336 reacts with a common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex.
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Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
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Species reactivityReacts with: Mouse
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Immunogen
TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)
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General notes
ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
PurityAffinity purified
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Purification notesPurified from tissue culture supernatant
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Primary antibody notesab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.
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ClonalityMonoclonal
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Clone numberH57-597
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab25336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IF | Use at an assay dependent concentration. |
Target
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RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
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Cellular localizationType I membrane protein.
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Database links
- Entrez Gene: 21473 Mouse
- Entrez Gene: 21577 Mouse
- SwissProt: P01849 Mouse
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Alternative names
- FLJ22602 antibody
- MGC117436 antibody
- MGC22624 antibody
see all
References
This product has been referenced in:
- Liang W et al. Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation. Front Immunol 9:78 (2018). Read more (PubMed: 29434598) »
- Shi YF et al. In vivo administration of monoclonal antibodies to the CD3 T cell receptor complex induces cell death (apoptosis) in immature thymocytes. J Immunol 146:3340-6 (1991). Read more (PubMed: 1827483) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"