Overview

  • Product name
    Anti-TCR alpha + TCR beta antibody [H57-597] (FITC)
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Armenian hamster monoclonal [H57-597] to TCR alpha + TCR beta (FITC)
  • Host species
    Armenian hamster
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)

  • General notes

    ab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
  • Storage buffer
    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Purified from TCS
  • Primary antibody notes
    ab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells.
  • Clonality
    Monoclonal
  • Clone number
    H57-597
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance
    The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localization
    Type I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Images

  • Flow Cytometry analysis of C57Bl/6 splenocytes labeling TCR alpha + TCR beta with ab25010 at 0.25µg (solid line). Armenian hamster IgG (FITC) isotype control at 0.25µg (dashed line).

References

This product has been referenced in:
  • Kubo RT  et al. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol 142:2736-42 (1989). Read more (PubMed: 2467936) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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