Product nameAnti-TCR alpha antibody
See all TCR alpha primary antibodies
DescriptionRabbit polyclonal to TCR alpha
Tested applicationsSuitable for: IHC-P, WB, IP, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein (Human)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab18861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.
Cellular localizationPlasma membrane
- T cell antigen receptor alpha polypeptide antibody
- T cell receptor alpha antibody
- T cell receptor alpha locus antibody
All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
TCR alpha contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.