- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TCR alpha antibody
See all TCR alpha primary antibodies -
DescriptionRabbit polyclonal to TCR alpha
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, IP, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant full length protein (Human)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab18861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | ||
| WB | ||
| IP | ||
| IHC-Fr |
Application notes
IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceTCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.
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Cellular localizationPlasma membrane
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Database links
- Entrez Gene: 6955 Human
- Entrez Gene: 100101484 Mouse
- Omim: 186880 Human
- SwissProt: P01848 Human
- SwissProt: P01849 Mouse
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Alternative names
- T cell antigen receptor alpha polypeptide antibody
- T cell receptor alpha antibody
- T cell receptor alpha locus antibody
see all
Images
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Western blot - Anti-TCR alpha antibody (ab18861)All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 21 kDa (why is the actual band size different from the predicted?)
Additional bands at: 56 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
TCR alpha contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References
This product has been referenced in:
- Liu Y et al. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1(+)Treg cells. Nat Commun 8:14709 (2017). IF ; Mouse . Read more (PubMed: 28436428) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"