Overview

  • Product name
  • Description
    Rabbit polyclonal to TCR alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab18861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
IP
IHC-Fr
  • Application notes
    IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.
    • Cellular localization
      Plasma membrane
    • Database links
    • Alternative names
      • T cell antigen receptor alpha polypeptide antibody
      • T cell receptor alpha antibody
      • T cell receptor alpha locus antibody
      • T-cell receptor alpha chain C region antibody
      • TCRA antibody
      • TCRD antibody
      • TRA antibody
      • TRA@ antibody
      • TRAC antibody
      see all

    Images

    • All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml

      Lane 1 : Human thymus tissue lysate - total protein (ab30146)
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 16 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 56 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 90 seconds


      TCR alpha contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    References

    This product has been referenced in:
    • Liu Y  et al. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1(+)Treg cells. Nat Commun 8:14709 (2017). IF ; Mouse . Read more (PubMed: 28436428) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Blocking step
    BSA in FCS (20ml) + DMEM (80ml) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

    Dr. James Jupp

    Verified customer

    Submitted Nov 06 2009

    Answer

    Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in these applications, and I am sorry this means I am unable to supply you with further details on FACS or its functionality as a stimulating antibody. All tested applications are specified on Abcam product datasheets. As this antibody is a polyclonal antibody to the whole human recombinant protein, it will recognise many epitopes on the protein. If you require more specificity on the epitope, you may like to try a monoclonal antibody. If you decide to go ahead and purchase this product for these new applications, I would be very pleased to hear how you get on . We would be very grateful if you could submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. If you send us an Abreview, we can offer you from 50 up to 300 Abcam points (Abpoints) based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. Furthermore, you get 100 extra Abpoints for submitting an image. These Abpoints can then be claimed back as rewards. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    ELISA
    Sample
    Not applicable Recombinant protein (Human/mouse chimeric alpha/beta TCR)
    Specification
    Human/mouse chimeric alpha/beta TCR
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
    Type
    Sandwich

    Abcam user community

    Verified customer

    Submitted Apr 19 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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