Anti-TCR alpha antibody (ab18861)

Reviews (4)Q&A (1)References (1)
Western blot - Anti-TCR alpha antibody (ab18861)

    Key features and details

    • Rabbit polyclonal to TCR alpha
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-TCR alpha antibody
      See all TCR alpha primary antibodies

    • Description

      Rabbit polyclonal to TCR alpha

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Recombinant full length protein (Human)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18861 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (2)
    Notes
  • Application notes
    IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.

    • Cellular localization

      Plasma membrane

    • Database links

      • Alternative names

        • T cell antigen receptor alpha polypeptide antibody
        • T cell receptor alpha antibody
        • T cell receptor alpha locus antibody
        • T-cell receptor alpha chain C region antibody
        • TCRA antibody
        • TCRD antibody
        • TRA antibody
        • TRA@ antibody
        • TRAC antibody
        see all

      Images

      • Western blot - Anti-TCR alpha antibody (ab18861)
        Western blot - Anti-TCR alpha antibody (ab18861)
        All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml

        Lane 1 : Human thymus tissue lysate - total protein (ab30146)
        Lane 2 : Human spleen tissue lysate - total protein (ab29699)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 16 kDa
        Observed band size: 21 kDa why is the actual band size different from the predicted?
        Additional bands at: 56 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 90 seconds


        TCR alpha contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      References (1)

      Publishing research using ab18861? Please let us know so that we can cite the reference in this datasheet.

      ab18861 has been referenced in 1 publication.

      • Liu Y  et al. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1(+)Treg cells. Nat Commun 8:14709 (2017). IF ; Mouse . PubMed: 28436428

      Customer reviews and Q&As

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      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-TCR alpha antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Dopaminergic Neurons)
      Gel Running Conditions
      Reduced Denaturing (NuPage 4-12% MES buffer)
      Loading amount
      30 µg
      Specification
      Dopaminergic Neurons
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 22 2020

      Western blot abreview for Anti-TCR alpha antibody

       Below Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (colon cancer)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      colon cancer
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 09 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TCR alpha antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Tonsil)
      Specification
      Tonsil
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate
      Permeabilization
      No
      Blocking step
      BSA in FCS (20ml) + DMEM (80ml) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
      Read More

      Dr. James Jupp

      Verified customer

      Submitted Nov 06 2009

      Question

      I am interesting in using your anti-TCRalpha polyclonal antibody, but I need to know if you have some information regarding its funcionality in FACS or as stimulating antibody. Also I would like to ask if you know the epitope in TCRalpha that is recognized by this antibody.

      Read More
      Answer

      Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in these applications, and I am sorry this means I am unable to supply you with further details on FACS or its functionality as a stimulating antibody. All tested applications are specified on Abcam product datasheets. As this antibody is a polyclonal antibody to the whole human recombinant protein, it will recognise many epitopes on the protein. If you require more specificity on the epitope, you may like to try a monoclonal antibody. If you decide to go ahead and purchase this product for these new applications, I would be very pleased to hear how you get on . We would be very grateful if you could submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. If you send us an Abreview, we can offer you from 50 up to 300 Abcam points (Abpoints) based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. Furthermore, you get 100 extra Abpoints for submitting an image. These Abpoints can then be claimed back as rewards. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      ELISA abreview for Anti-TCR alpha antibody

       Poor
      Abreviews
      Application
      ELISA
      Sample
      Not applicable Recombinant protein (Human/mouse chimeric alpha/beta TCR)
      Specification
      Human/mouse chimeric alpha/beta TCR
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
      Type
      Sandwich
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 19 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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