PE Anti-TCR alpha + TCR beta antibody [IP26] (ab99857)
Key features and details
- PE Mouse monoclonal [IP26] to TCR alpha + TCR beta
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-TCR alpha + TCR beta antibody [IP26]
See all TCR alpha + TCR beta primary antibodies -
Description
PE Mouse monoclonal [IP26] to TCR alpha + TCR beta -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
Recognizes a monomorphic determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human TCR alpha + TCR beta
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Positive control
- Human peripheral blood T cells
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General notes
Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. No reconstitution is necessary.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
IP26 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
or 100 µl of whole blood. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. or 100 µl of whole blood. |
Target
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Relevance
The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 6955 Human
- Entrez Gene: 6957 Human
- Omim: 186880 Human
- Omim: 186930 Human
- SwissProt: P01848 Human
- SwissProt: P04435 Human
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Alternative names
- FLJ22602 antibody
- MGC117436 antibody
- MGC22624 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab99857 has been referenced in 1 publication.
- Kuttruff S et al. NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood 113:358-69 (2009). PubMed: 18922855