Overview

  • Product name

    Anti-TCR alpha + TCR beta antibody [R73]
    See all TCR alpha + TCR beta primary antibodies
  • Description

    Mouse monoclonal [R73] to TCR alpha + TCR beta
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Fr, Functional Studies, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Non human primates
  • Immunogen

    Rat T blast cells and erythrocytes.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purity: > 95% (by SDS-PAGE).
  • Clonality

    Monoclonal
  • Clone number

    R73
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localization

    Type I membrane protein.
  • Database links

  • Alternative names

    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Images

  • ab139367 staining TCR alpha + TCR beta in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 1 hour at 22°C. A Cy3®-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C

Mr. Thomas Schöppe

Verified customer

Submitted Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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