Overview

  • Product name

    Anti-TCR beta F1 antibody [8A3]
  • Description

    Mouse monoclonal [8A3] to TCR beta F1
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human, Non human primates
  • Immunogen

    Full length protein corresponding to Human TCR beta F1.
    Database link: P04435

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.5% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    8A3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Target

  • Cellular localization

    Plasma membrane
  • Database links

  • Alternative names

    • T Cell Receptor Beta Locus antibody
    • T-Cell Antigen Receptor, Beta Polypeptide, T-Cell Receptor, Beta Cluster antibody
    • T-cell receptor beta chain V region CTL-L17 antibody
    • T-Cell Receptor, Beta Cluster antibody
    • TCRB antibody
    • TRB antibody
    see all

Images

  • Flow cytometry analysis of TCR beta F1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR beta F1 monoclonal antibody (ab171088) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

References

ab171088 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil, thymoma)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K, 5 min, 37C
Permeabilization
No
Specification
tonsil, thymoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 16 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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