Product nameAnti-TCR beta F1 antibody [8A3]
DescriptionMouse monoclonal [8A3] to TCR beta F1
Tested applicationsSuitable for: ICC, IHC-P, WB, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Human, Non human primates
Full length protein corresponding to Human TCR beta F1.
Database link: P04435
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.5% BSA, 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab171088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
Cellular localizationPlasma membrane
- T Cell Receptor Beta Locus antibody
- T-Cell Antigen Receptor, Beta Polypeptide, T-Cell Receptor, Beta Cluster antibody
- T-cell receptor beta chain V region CTL-L17 antibody
Flow cytometry analysis of TCR beta F1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR beta F1 monoclonal antibody (ab171088) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
ab171088 has not yet been referenced specifically in any publications.