FITC Anti-TCR gamma + TCR delta antibody [5A6.E91] (ab171110)
Key features and details
- FITC Mouse monoclonal [5A6.E91] to TCR gamma + TCR delta
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-TCR gamma + TCR delta antibody [5A6.E91]
See all TCR gamma + TCR delta primary antibodies -
Description
FITC Mouse monoclonal [5A6.E91] to TCR gamma + TCR delta -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human TCR gamma + TCR delta. Native protein
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.5% BSA, Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5A6.E91 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/20.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
1/20. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. -
Cellular localization
Type I membrane protein -
Database links
- Entrez Gene: 6964 Human
- Entrez Gene: 6965 Human
- Omim: 186810 Human
- Omim: 186970 Human
- SwissProt: B7Z8K6 Human
- SwissProt: P03986 Human
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Alternative names
- CD_antigen=CD3d antibody
- CD3D antibody
- CD3G antibody
see all
Images
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Flow cytometry analysis of TCR gamma + TCR delta showing positive staining in the membrane of peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected and combined with a hydrophilic polysaccharide then centrifuged. Samples transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml and 50 ul of isotype control and ab171110 at 1:20 added. Cells were incubated for 30 min at 4°C and washed with a cell buffer and incubated with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
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Flow cytometry analysis of TCR gamma + TCR delta showing positive staining in the membrane of peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected and combined with a hydrophilic polysaccharide then centrifuged. Samples were transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml and 50 ul of isotype control and ab171110 at 1:20 added. Cells were incubated for 30 min at 4°C and washed with a cell buffer and incubated with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab171110 has been referenced in 1 publication.
- Wang J et al. Tumor-infiltrating ?dT cells predict prognosis and adjuvant chemotherapeutic benefit in patients with gastric cancer. Oncoimmunology 6:e1353858 (2017). PubMed: 29147601