Product nameAnti-TCR gamma + TCR delta antibody [UC7-13D5] (PE)
See all TCR gamma + TCR delta primary antibodies
DescriptionArmenian hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta (PE)
Host speciesArmenian hamster
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab25663 is specific for the gamma-delta heterodimer and plate-bound ab25663 activates gamma-delta TRC-bearing cells. This antibody does not react with alpha-beta TCR-expressing T cells.
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Cellular Activationmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
Storage instructionsStore at +4°C in the dark. Do not freeze.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab25663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cellular depletion (In vivo depletion of γδ TCR-expressing T cells): Use at an assay dependent dilution.
Flow Cyt: Use 0.3µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceT cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease.
Cellular localizationType I membrane protein
- CD_antigen=CD3d antibody
- CD3D antibody
- CD3G antibody
ab25663 has been referenced in 4 publications.
- Bluestone JA et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol Rev 120:5-33 (1991). PubMed: 1650760
- Barrett TA et al. Control of self-reactivity in the intestine. Immunol Res 10:341-8 (1991). PubMed: 1835488
- Dent AL et al. Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343:714-9 (1990). PubMed: 1968227
- Houlden BA et al. A TCR gamma delta cell recognizing a novel TL-encoded gene product. Cold Spring Harb Symp Quant Biol 54 Pt 1:45-55 (1989). PubMed: 2576978