Anti-TCR gamma + TCR delta antibody [5A6.E91], prediluted (FITC) (ab171110)

Overview

  • Product name
    Anti-TCR gamma + TCR delta antibody [5A6.E91], prediluted (FITC)
    See all TCR gamma + TCR delta primary antibodies
  • Description
    Mouse monoclonal [5A6.E91] to TCR gamma + TCR delta, prediluted (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ELISA, Blocking, ICC, IHC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Non human primates
  • Immunogen

    Full length protein corresponding to Human TCR gamma + TCR delta.

Properties

Applications

Our Abpromise guarantee covers the use of ab171110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC Use at an assay dependent concentration.
Flow Cyt 1/20.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease.
  • Cellular localization
    Type I membrane protein
  • Database links
  • Alternative names
    • CD_antigen=CD3d antibody
    • CD3D antibody
    • CD3G antibody
    • T cell antigen receptor delta polypeptide antibody
    • T cell antigen receptor gamma polypeptide antibody
    • T cell rearranging gene gamma antibody
    • T cell receptor delta antibody
    • T cell receptor delta locus antibody
    • T cell receptor gamma antibody
    • T cell receptor gamma chain C region PT gamma 1/2 antibody
    • T cell receptor gamma cluster antibody
    • T cell receptor gamma locus antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor, delta (V,D,J,C) antibody
    • T cell receptors antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3D antibody
    • T3G antibody
    • TCRD antibody
    • TCRDV1 antibody
    • TCRG antibody
    • TRD antibody
    • TRD locus antibody
    • TRD@ antibody
    • TRG antibody
    • TRG locus antibody
    • TRGC2 antibody
    see all

Images

  • Flow cytometry analysis of TCR gamma + TCR delta showing positive staining in the membrane of peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected and combined with a hydrophilic polysaccharide then centrifuged. Samples transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml and 50 ul of isotype control and ab171110 at 1:20 added. Cells were incubated for 30 min at 4�C and washed with a cell buffer and incubated with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4�C in the dark. FACS analysis was performed using 400 ul of cell buffer.
  • Flow cytometry analysis of TCR gamma + TCR delta showing positive staining in the membrane of peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected and combined with a hydrophilic polysaccharide then centrifuged. Samples were transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml and 50 ul of isotype control and ab171110 at 1:20 added. Cells were incubated for 30 min at 4�C and washed with a cell buffer and incubated with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4�C in the dark. FACS analysis was performed using 400 ul of cell buffer.

References

This product has been referenced in:
  • Wang J  et al. Tumor-infiltrating ?dT cells predict prognosis and adjuvant chemotherapeutic benefit in patients with gastric cancer. Oncoimmunology 6:e1353858 (2017). Read more (PubMed: 29147601) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171110.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up