Overview

  • Product name
    Anti-TCR V alpha 2 antibody [F1] (FITC)
    See all TCR V alpha 2 primary antibodies
  • Description
    Mouse monoclonal [F1] to TCR V alpha 2 (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Blocking, WB, IHC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Non human primates
  • Immunogen

    Full length protein corresponding to Human TCR V alpha 2.
    Database link: P01848

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.5% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    F1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
IHC Use at an assay dependent concentration.
Flow Cyt 1/10.

ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Cellular localization
    Membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • T cell receptor alpha locus antibody
    • T-cell antigen receptor, alpha polypeptide antibody
    • T-cell receptor alpha chain C region antibody
    • T-cell receptor, alpha (V,D,J,C) antibody
    • TCRA antibody
    • TCRD antibody
    • TRA@ antibody
    see all

Images

  • Flow cytometry analysis of TCR V alpha 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V alpha 2 monoclonal antibody (ab171102) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

References

ab171102 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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