Full length protein corresponding to Human TCR V alpha 2.
Database link: P01848
Our Abpromise guarantee covers the use of ab171102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.|
|IHC||Use at an assay dependent concentration.|
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Flow cytometry analysis of TCR V alpha 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V alpha 2 monoclonal antibody (ab171102) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
ab171102 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"