Product nameAnti-TCR V beta 3.1 antibody [8F10], prediluted (FITC)
See all TCR V beta 3.1 primary antibodies
DescriptionMouse monoclonal [8F10] to TCR V beta 3.1, prediluted (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Blocking, ELISA, IP, ICC, IHC, Flow Cytmore details
Species reactivityReacts with: Human, Non human primates
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.5% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab171112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 200 µg/ml.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
- T cell receptor beta locus antibody
- T-cell receptor beta chain V region CTL-L17 antibody
- TCRB antibody
- TRB antibody
Flow cytometry analysis of TCR V beta 3.1 in PBMC cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab171112) at a dilution of 1:20. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
ab171112 has not yet been referenced specifically in any publications.