Anti-TCR V beta 5 antibody [MEM-262] (ab1898)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-TCR V beta 5 antibody [MEM-262]
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DescriptionMouse monoclonal [MEM-262] to TCR V beta 5
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Host speciesMouse
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SpecificityThe antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
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Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Human thymoma cell line HPB-ALL.
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General notesFunctional Application Activates T cells (V beta 5-related subset).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
Purity>95% by SDS-PAGE
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Purification notesPurified from hybridoma culture supernatant by protein A affinity chromatography.
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ClonalityMonoclonal
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Clone numberMEM-262
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | ||
| IP | ||
| WB |
Application notes
Flow Cyt: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceT cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.
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Cellular localizationCell Membrane
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Database links
- Entrez Gene: 28612 Human
- Omim: 186930 Human
- SwissProt: P03979 Human
- SwissProt: P04435 Human
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Alternative names
- T cell receptor antibody
- TCB antibody
- TCRB antibody
see all
References
This product has been referenced in:
- Wang CT et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). Read more (PubMed: 25839766) »
- Grabherr MG et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). Read more (PubMed: 21625601) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"