Anti-TCR V beta 5 antibody [MEM-262] (ab1898)

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Overview

  • Product name

    Anti-TCR V beta 5 antibody [MEM-262]

  • Description

    Mouse monoclonal [MEM-262] to TCR V beta 5

  • Host species

    Mouse

  • Specificity

    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Human thymoma cell line HPB-ALL.

  • General notes

    Functional Application Activates T cells (V beta 5-related subset).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    MEM-262

  • Isotype

    IgG2a

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.

Target

  • Relevance

    T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    References

    This product has been referenced in:

    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). Read more (PubMed: 25839766) »
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). Read more (PubMed: 21625601) »
    See all 3 Publications for this product

    Publishing research using ab1898? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example Hela or T24, which was used for immunization of the mice) as a positive control in WB. You may also want to refer to the following publications for additional information about this antibody: Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512 Schaafsma HE et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136:329-43 (1990). PubMed: 1689541 If you have any additional questions, please contact us again.

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    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa The following publication has used this antibody and you may find some additional here as well: Pavlistová D et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678 I have not yet received information regarding ab9219 and will contact you again when I do.

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