Anti-TCR V beta 5 antibody [MEM-262] (ab1898)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [MEM-262] to TCR V beta 5
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-TCR V beta 5 antibody [MEM-262]

  • Description

    Mouse monoclonal [MEM-262] to TCR V beta 5

  • Host species

    Mouse

  • Specificity

    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Human thymoma cell line HPB-ALL.

  • General notes

    Functional Application Activates T cells (V beta 5-related subset).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    MEM-262

  • Isotype

    IgG2a

  • Research areas

Target

  • Relevance

    T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    References (3)

    Publishing research using ab1898? Please let us know so that we can cite the reference in this datasheet.

    ab1898 has been referenced in 3 publications.

    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). PubMed: 25839766
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601
    • Pavlistová D  et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example Hela or T24, which was used for immunization of the mice) as a positive control in WB. You may also want to refer to the following publications for additional information about this antibody: Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512 Schaafsma HE et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136:329-43 (1990). PubMed: 1689541 If you have any additional questions, please contact us again.

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    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa The following publication has used this antibody and you may find some additional here as well: Pavlistová D et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678 I have not yet received information regarding ab9219 and will contact you again when I do.

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