Anti-TCR V beta 5a antibody [1C1] (FITC) (ab171098)

Overview

  • Product name
    Anti-TCR V beta 5a antibody [1C1] (FITC)
    See all TCR V beta 5a primary antibodies
  • Description
    Mouse monoclonal [1C1] to TCR V beta 5a (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Cellular Activation, Blocking, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human, Non human primates
  • Immunogen

    Full length protein corresponding to Human TCR V beta 5a.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: Glycerol, 0.5% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    1C1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Cellular Activation Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

2 μg/test

 

 

 

 

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Database links
  • Alternative names
    • T cell receptor beta locus antibody
    • T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster antibody
    • T-cell receptor beta chain V region CTL-L17 antibody
    • T-cell receptor, beta cluster antibody
    • TCRB antibody
    • TRB antibody
    • TRB@ antibody
    see all

Images

  • Flow cytometry analysis of TCR V beta 5a in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab171098) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.

  • Flow cytometry analysis of TCR V beta 5a in mouse splenocytes (green) compared to an isotype control (blue). Spleen cells were lysed and diluted and 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml. 50 ul of isotype control and primary antibody (ab171098) at a dilution of 2 ug/test were added and incubated for 30 min at room temperature. Cells were then incubated for 30 min at 4ºC in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

References

ab171098 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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