Overview

  • Product name
    Anti-TCR V delta 1 antibody [TS8.2]
    See all TCR V delta 1 primary antibodies
  • Description
    Mouse monoclonal [TS8.2] to TCR V delta 1
  • Host species
    Mouse
  • Tested applications
    Suitable for: Cellular Activation, ELISA, ICC, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human, Non human primates
  • Immunogen

    Full length protein corresponding to Human TCR V delta 1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    TS8.2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Cellular Activation Use at an assay dependent concentration.

T cell Activation

ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

2 μg/ Test

 

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Two distinct types of T cell antigen receptors have been identified: the alpha/beta heterodimer found on functional helper and cytotoxic T cells, and the gamma/delta heterodimer. The latter is first detected approximately 2 days before the appearance of cell surface alpha/beta heterodimer during T cell ontogeny. In adult thymus it is found mainly in the least mature cells. The gene shows systematic rearrangement in early thymocytes and appears to use V gene segments of the TCR alpha chain family, well before any C(alpha) message of protein is detected. RNA from this locus is expressed at a high level in early thymocytes and adult 'double-negative' cells, but not in mature T cell populations.
  • Database links
  • Alternative names
    • T cell antigen receptor delta polypeptide antibody
    • T cell receptor delta (V,D,J,C) antibody
    • T cell receptor delta locus antibody
    • TCRD antibody
    • TCRDV1 antibody
    • TRA@ protein antibody
    • TRD antibody
    • TRD@ antibody
    see all

Images

  • Flow cytometry analysis of TCR V delta 1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V delta 1 monoclonal antibody (ab171113) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

References

ab171113 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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