Key features and details
- Mouse monoclonal [15D] to TCR V delta 2
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Drosophila melanogaster, Non human primates, Rhesus monkey
- Isotype: IgG1
Product nameAnti-TCR V delta 2 antibody [15D]
DescriptionMouse monoclonal [15D] to TCR V delta 2
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human, Drosophila melanogaster, Non human primates, Rhesus monkey
Full length protein corresponding to Human TCR V delta 2.
Database link: 6964
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab171103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
- TCRD antibody
- TCRDV1 antibody
- TRD@ antibody
Western blot analysis of TCR V delta 2 was performed by loading 25 ug of Jurkat (lane 1) and CEM (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V delta 2 monoclonal antibody (ab171103) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using ECL Plus.Results show a band at ~19 kDa.
Flow cytometry analysis of TCR V delta 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V delta 2 monoclonal antibody (ab171103) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
ab171103 has been referenced in 1 publication.
- Yin S et al. Hyperactivation and in situ recruitment of inflammatory Vd2 T cells contributes to disease pathogenesis in systemic lupus erythematosus. Sci Rep 5:14432 (2015). PubMed: 26395317