Full length protein corresponding to Human TCR V gamma 9.
Database link: 6965
Our Abpromise guarantee covers the use of ab171109 in the following tested applications.
|WB||1/1 - 1/100.|
|Cellular Activation||Use at an assay dependent concentration.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Flow cytometry analysis of TCR V gamma 9 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V gamma 9 monoclonal antibody (ab171109) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis
Western blot analysis of TCR V gamma 9 was performed by loading 25 ug of CEM cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V gamma 9 monoclonal antibody (ab171109) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using an ECL Plus Western Blotting Substrate.Results show a band at ~18-28 kDa.
ab171109 has not yet been referenced specifically in any publications.