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I have checked with customer and then informed as as follows:
First we treated antibody with modifier reagent and then this mixture in your Easylink Conjugate.
In this labelling reaction, after incubation quencher is added.
Asked on Jun 18 2012
Thank you for your answer and confirming that your customer added the modifier. The modifier step is inherent to the protocol.
I have to repeat that this kit can only be used for purified antibodies. The antibodies need to be purified before the conjugation reaction.
ab102885 will label any available amine with FITC. ab91655 is not purified and therefore contains a lot of proteins that have free amines. All these free amines will interfere with the conjugation of the antibody.
I would like to summarize that ab102885 has to be purified before being labeled with the EasyLink FITC Conjugation Kit. The purification can be done with ab109207 (Antibody TCS Purification Kit):
I hope this information is helpful for your customer.
Answered on Jun 18 2012