Product nameTCS Antibody Purification System - AbPure™
TCS Antibody Purification System - AbPure™ (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants. AbPure™ Protein A resin is prepared by coupling purified Protein A to agarose beads.
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ TCS Antibody Purification System. Expedeon product code 264-0030 is the same as the 1 pack size of this product and code 264-0500 is the same as the 3 pack size.
The method involves capture of the antibody on the AbPure™ resin and the removal of unwanted substances using a simple wash procedure. The antibody is then eluted and neutralized. The kit can be used in its rapid version by spin-purification in a centrifuge, or by gravity-flow purification. The antibody to be purified should be in 10 to 50 mL of tissue culture supernatant (TCS). Up to 5 mg of antibody can be purified in each run.
This kit is fully compatible with our Gold and the Latex conjugation kits (available separately), which allow the purified antibody to be conjugated rapidly.
- Easy and quick to use
- High recoveries
- Specifically developed for use with our Latex and Gold conjugation kits
Storage instructionsPlease refer to protocols.
Components 1 packs 3 packs 10X AbPureTM Binding Buffer 1 unit 1 unit AbPureTM Elution Buffer 1 unit 1 unit AbPureTM Neutralization Buffer 1 unit 1 unit AbPureTM Preservative 1 unit 1 unit AbPureTM TCS Protein A resin 1 unit 3 units AbPureTM Wash Buffer 1 unit 1 unit Collection Tubes (15 mL) 4 units 12 units Concentrator Spin Columns 1 unit 3 units Spin Purification Columns 1 unit 3 units
ab270554 has not yet been referenced specifically in any publications.