Key features and details
- Rabbit polyclonal to TCTA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCTA antibody
See all TCTA primary antibodies
DescriptionRabbit polyclonal to TCTA
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
- WB: Mouse liver, gonadal and muscle tissue lysate; HL-60, U-251 MG and MCF7 whole cell lysate. IHC-P: Human adrenal gland and colon cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IHC-P||1/20 - 1/200.|
FunctionMay be required for cellular fusion during osteoclastogenesis.
Tissue specificityUbiquitous. Highest level of expression in kidney. Present in monocytes, osteoclasts, macrophages, synoviocytes and synovial lining cells (at protein level).
Involvement in diseaseA chromosomal aberration involving TCTA is associated with T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(1;3)(p34;p21).
Sequence similaritiesBelongs to the TCTA family.
- Information by UniProt
- T-cell leukemia translocation altered gene antibody
- T-cell leukemia translocation-altered gene protein antibody
- T-cell leukemia translocation-associated gene protein antibody
All lanes : Anti-TCTA antibody (ab235781) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse gonadal tissue lysate
Lane 3 : Mouse muscle tissue lysate
Lane 4 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 5 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Paraffin-embedded human adrenal gland tissue stained for TCTA using ab235781 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for TCTA using ab235781 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235781 has not yet been referenced specifically in any publications.