Key features and details
- Rabbit polyclonal to TCTEX-1 - Membrane Vesicle Marker
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCTEX-1 antibody - Membrane Vesicle Marker
See all TCTEX-1 primary antibodies
DescriptionRabbit polyclonal to TCTEX-1 - Membrane Vesicle Marker
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK1, and NIH 3T3 whole cell lysates.
This product was previously labelled as DYNLT1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab206683 was affinity purified using an epitope specific to TCTEX-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab206683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Predicted molecular weight: 12 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein.
Plays a role in neuronal morhpogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation.
Tissue specificityExpressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).
Sequence similaritiesBelongs to the dynein light chain Tctex-type family.
modificationsPhosphorylated by BMPR2; the phosphorylation is abolished by BMPR2 mutations in exon 12 which lead to truncated forms of BMPR2 and which are linked to primary pulmonary hypertension (PPH1) [MIM:178600]. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release.
Cellular localizationGolgi apparatus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to mitotic spindles.
- Information by UniProt
- AGS2 antibody
- CW 1 antibody
- DYLT1_HUMAN antibody
All lanes : Anti-TCTEX-1 antibody - Membrane Vesicle Marker (ab206683) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 12 kDa
Exposure time: 30 seconds
Detection of TCTEX-1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206683 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds
ab206683 has not yet been referenced specifically in any publications.