Overview

  • Product name

    Anti-TCTEX-1 antibody - Membrane Vesicle Marker
    See all TCTEX-1 primary antibodies
  • Description

    Rabbit polyclonal to TCTEX-1 - Membrane Vesicle Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human TCTEX-1 aa 1-50 (N terminal). The exact sequence is proprietary.
    Sequence:

    MEDYQAAEET AFVVDEVSNI VKEAIESAIG GNAYQHSKVN QWTTNVVEQT


    Database link: P63172

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1, and NIH 3T3 whole cell lysates.
  • General notes

     This product was previously labelled as DYNLT1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab206683 was affinity purified using an epitope specific to TCTEX-1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000. Predicted molecular weight: 12 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein.
    Plays a role in neuronal morhpogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation.
  • Tissue specificity

    Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).
  • Sequence similarities

    Belongs to the dynein light chain Tctex-type family.
  • Post-translational
    modifications

    Phosphorylated by BMPR2; the phosphorylation is abolished by BMPR2 mutations in exon 12 which lead to truncated forms of BMPR2 and which are linked to primary pulmonary hypertension (PPH1) [MIM:178600]. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release.
  • Cellular localization

    Golgi apparatus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to mitotic spindles.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGS2 antibody
    • CW 1 antibody
    • DYLT1_HUMAN antibody
    • Dynein light chain Tctex-type 1 antibody
    • DYNLT1 antibody
    • MGC111571 antibody
    • Protein CW-1 antibody
    • RP11-114M11.1 antibody
    • T complex associated testis expressed 1 like 1 antibody
    • T-complex testis-specific protein 1 homolog antibody
    • TCTEL1 antibody
    • tctex 1 antibody
    • Tctex1 antibody
    see all

Images

  • All lanes : Anti-TCTEX-1 antibody - Membrane Vesicle Marker (ab206683) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 12 kDa


    Exposure time: 30 seconds
  • Detection of TCTEX-1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206683 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds

References

ab206683 has not yet been referenced specifically in any publications.

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