Key features and details
- Rabbit polyclonal to TCTN2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCTN2 antibody
DescriptionRabbit polyclonal to TCTN2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human fallopian tube tissue. WB: TCTN2 overexpression HEK-293T lysate. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243814 in the following tested applications.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 77 kDa.|
Involvement in diseaseDefects in TCTN2 are the cause of Meckel syndrome type 8 (MKS8) [MIM:613885]. A disorder characterized by a combination of renal cysts and variably associated features including developmental anomalies of the central nervous system (typically encephalocele), hepatic ductal dysplasia and cysts, and polydactyly.
Sequence similaritiesBelongs to the tectonic family.
- Information by UniProt
- C12orf38 antibody
- FLJ12975 antibody
- MKS8 antibody
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling TCTN2 using ab243814 at 4 µg/ml (green) in ICC/IF.
All lanes : Anti-TCTN2 antibody (ab243814) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : TCTN2 overexpression HEK-293T lysate
Predicted band size: 77 kDa
Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for TCTN2 with ab243814 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for TCTN2 with ab243814 at a 1/200 dilution in immunohistochemical analysis. Low expression as expected.
ab243814 has not yet been referenced specifically in any publications.