Key features and details
- Rabbit polyclonal to TCTN3
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-TCTN3 antibody
DescriptionRabbit polyclonal to TCTN3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human TCTN3 aa 348-472.
Database link: Q6NUS6
- WB: HEK-293, A549 and HepG2 whole cell lysates; Rat heart, liver and stomach tissue lysates. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/50 - 1/500.|
FunctionMay be involved in apoptosis regulation.
Sequence similaritiesBelongs to the tectonic family.
- Information by UniProt
- C10orf61 antibody
- Chromosome 10 open reading frame 61 antibody
- DKFZP564D116 antibody
All lanes : Anti-TCTN3 antibody (ab237512) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 6 : Rat stomach tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TCTN3 (green) using ab237512 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237512 has not yet been referenced specifically in any publications.