Overview

  • Product name
  • Description
    Rabbit polyclonal to TCTP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Rabbit, Chicken, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TCTP.

    Read Abcam's proprietary immunogen policy (Peptide available as ab37505.)

  • Positive control
    • Recombinant Human TCTP protein (ab93454) can be used as a positive control in WB. This antibody gave a positive control in the follwing Human whole cell lysates: HeLa, Jurkat, A431, HEK 293, HepG2, MCF-7 and U2OS; Mouse whole cell lysates: NIH 3T3 and MEF1; Mouse tissue lysates: Pancreas, Testis, Spinal Cord and Ovary; Rat whole cell lysate: PC12; Rat tissue lysate: Liver

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/2000.

Target

Images

  • All lanes : Anti-TCTP antibody (ab37506) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)
    Lane 5 : HepG2 whole cell lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-TCTP antibody (ab37506) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 5 : Spinal Cord (Mouse) Tissue Lysate
    Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 8 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Bae SY  et al. Immunohistochemical Localization of Translationally Controlled Tumor Protein in Axon Terminals of Mouse Hippocampal Neurons. Exp Neurobiol 26:82-89 (2017). IHC-P ; Mouse . Read more (PubMed: 28442944) »
  • Bommer UA  et al. Translationally controlled tumour protein TCTP is induced early in human colorectal tumours and contributes to the resistance of HCT116 colon cancer cells to 5-FU and oxaliplatin. Cell Commun Signal 15:9 (2017). Read more (PubMed: 28143584) »
See all 18 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your inquiry.

We have four polyclonal anti TCTP antibodies in our catalog, three are currently tested and guaranteed for WB.

In order to help you with your inquiry I will need the product number (starts with ab and then a number).

https://www.abcam.com/Search?Keywords=TCTP+&fReset=1&pt=-1&source=TopSearch&btnSearch=Search#Search?AppliedFacets.FacetClonality=Polyclonal&IsFacetsOnly=False&PageNumber=1&Keywords%5B0%5D=TCTP&X-Requested-With=XMLHttpRequest

The datasheet of our product usually also provide more information about the recommended dilutions for the tested applications etc.

We do not sell directly to Turkey. Please contact our trusted distributor for price information:

Kimera Medical Laboratory Supplies
Karadeniz Mh. General Aldogan Cad.
1154 SkNo:8 D:2, Gaziosmanpasa
Istanbul
34250
Turkey
Tel +90 2126493699 Fax +90 2124772985 Email mailto:info@kimeramolekuler.com



I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cell (Hep G2)
Specification
Hep G2
Permeabilization
Yes - triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Madin Darby Bovine Kidney cells)
Loading amount
100000 cells
Specification
Madin Darby Bovine Kidney cells
Treatment
Drug treatment and virus infection
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 16 2012

Answer

Thank you for contacting usyesterday and sorry for the delay in getting back to you.

As discussed over the phone, the anti-NTCTP antibody (ab37506) has not as yet been used in detecting thebovine protein as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet. As the immunogen used to raise this antibody shares 100% homology with thebovine protein we would expect the antibody to work withbovine samples.

As discussedover the phone, I am able to offer you a testing discount for this antibody. This offer means that if you test the antibodywith bovine samples and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab37506off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

As discussed over the phone, here is the discount code:

xxxxxxxxxxxxxx


Please quote this code in the "Additional comments" section when submitting your Abreview. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 19th ofNovember 2012.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your enquiry. We don't know of any published papers where this antibody has been used in IHC. However we do have 2 Abrevies, customer submitted reviews, linked to the datasheet for ab37506 in which the researchers used the antibody for IHC. These reviews contain protocol-specific details and images of the staining. To locate these reviews, just click on the 'Abreviews' tab on the online datsheet for ab37506. I hope this is helpful. Please contact me again if you have any further questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Genitourinary section)
Specification
Genitourinary section
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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