Overview

  • Product name

    Anti-TCTP antibody [EP5541]
    See all TCTP primary antibodies
  • Description

    Rabbit monoclonal [EP5541] to TCTP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human TCTP protein (P13693).

  • Positive control

    • Raji, LnCaP, Jurkat, and HepG2 cell lysates and Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.21 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Purification notes

    Not purified; provided in cell supernatant and storage buffer.
  • Clonality

    Monoclonal
  • Clone number

    EP5541
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Involved in calcium binding and microtubule stabilization.
    • Tissue specificity

      Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
    • Sequence similarities

      Belongs to the TCTP family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ27337 antibody
      • Fortilin antibody
      • Histamine releasing factor antibody
      • Histamine-releasing factor antibody
      • HRF antibody
      • Immunoglobulin E-dependent antibody
      • p02 antibody
      • p23 antibody
      • TCTP antibody
      • TCTP_HUMAN antibody
      • tpt1 antibody
      • Translationally controlled tumor protein antibody
      • Translationally-controlled tumor protein antibody
      • Tumor protein translationally controlled 1 antibody
      • tumor protein, translationally-controlled 1 antibody
      see all

    Images

    • All lanes : Anti-TCTP antibody [EP5541] (ab124835) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells using anti-TCTP in red (ab124835) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124835 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124835.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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