Recombinant
RabMAb

Recombinant Anti-TCTP antibody [EPR5540] (ab133568)

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Overview

  • Product name

    Anti-TCTP antibody [EPR5540]
    See all TCTP primary antibodies

  • Description

    Rabbit monoclonal [EPR5540] to TCTP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to a region of Human TCTP (P13693).

  • Positive control

    • A431 cells; Raji, HeLa, Jurkat and A431 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5540

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 20 kDa.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/10 - 1/100.
ICC/IF 1/250 - 1/500.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in calcium binding and microtubule stabilization.

  • Tissue specificity

    Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.

  • Sequence similarities

    Belongs to the TCTP family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ27337 antibody
    • Fortilin antibody
    • Histamine releasing factor antibody
    • Histamine-releasing factor antibody
    • HRF antibody
    • Immunoglobulin E-dependent antibody
    • p02 antibody
    • p23 antibody
    • TCTP antibody
    • TCTP_HUMAN antibody
    • tpt1 antibody
    • Translationally controlled tumor protein antibody
    • Translationally-controlled tumor protein antibody
    • Tumor protein translationally controlled 1 antibody
    • tumor protein, translationally-controlled 1 antibody
    see all

Images

  • Western blot - Anti-TCTP antibody [EPR5540] (ab133568)
    Western blot - Anti-TCTP antibody [EPR5540] (ab133568)
    All lanes : Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa

  • Flow Cytometry - Anti-TCTP antibody [EPR5540] (ab133568)
    Flow Cytometry - Anti-TCTP antibody [EPR5540] (ab133568)
    Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (ab133568)
    Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (ab133568)
    Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.

References

This product has been referenced in:

  • Neuhäuser K  et al. Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling. Clin Transl Radiat Oncol 15:99-107 (2019). Read more (PubMed: 30815593) »
  • Sun R  et al. TCTP promotes epithelial-mesenchymal transition in lung adenocarcinoma. Onco Targets Ther 12:1641-1653 (2019). Read more (PubMed: 30881019) »
See all 5 Publications for this product

Publishing research using ab133568? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-TCTP antibody [EPR5540]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CT26(1,2,3,4) / MC38(5,6,7,8))
Gel Running Conditions
Reduced Non-Denaturing (Native) (15% SDS PAGE GEL)
Loading amount
15 µg
Specification
CT26(1,2,3,4) / MC38(5,6,7,8)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 24 2018

Western blot abreview for Anti-TCTP antibody [EPR5540]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (15%)
Sample
Mouse Cell lysate - whole cell (MIN 6 cell)
Specification
MIN 6 cell
Treatment
transfect TCTP plasmid into MIN 6 cell for 24 hours
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Jan 23 2014

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