Recombinant Anti-TCTP antibody [EPR5540] (ab133568)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5540] to TCTP
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-TCTP antibody [EPR5540]
See all TCTP primary antibodies -
Description
Rabbit monoclonal [EPR5540] to TCTP -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to a region of Human TCTP (P13693).
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Positive control
- A431 cells; Raji, HeLa, Jurkat and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5540 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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ICC/IF |
Human
|
WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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WB | (2) |
1/10000 - 1/50000. Predicted molecular weight: 20 kDa.
|
Flow Cyt |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/250 - 1/500.
|
Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 20 kDa. |
Flow Cyt
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Involved in calcium binding and microtubule stabilization. -
Tissue specificity
Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate. -
Sequence similarities
Belongs to the TCTP family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7178 Human
- Entrez Gene: 22070 Mouse
- Entrez Gene: 116646 Rat
- Omim: 600763 Human
- SwissProt: P13693 Human
- SwissProt: P63028 Mouse
- SwissProt: P63029 Rat
- Unigene: 374596 Human
see all -
Alternative names
- FLJ27337 antibody
- Fortilin antibody
- Histamine releasing factor antibody
see all
Images
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All lanes : Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa -
Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab133568 has been referenced in 7 publications.
- D'Amico S et al. DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines. Cells 9:N/A (2020). PubMed: 32438775
- Neuhäuser K et al. Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling. Clin Transl Radiat Oncol 15:99-107 (2019). PubMed: 30815593
- Sun R et al. TCTP promotes epithelial-mesenchymal transition in lung adenocarcinoma. Onco Targets Ther 12:1641-1653 (2019). PubMed: 30881019
- Sun Y et al. miR-455-3p functions as a tumor suppressor in colorectal cancer and inhibits cell proliferation by targeting TPT1. Int J Clin Exp Pathol 11:2522-2529 (2018). PubMed: 31938365
- Pinkaew D et al. Fortilin binds IRE1a and prevents ER stress from signaling apoptotic cell death. Nat Commun 8:18 (2017). WB . PubMed: 28550308
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . PubMed: 25643152
- Chen K et al. Long-term artificial selection reveals a role of TCTP in autophagy in Mammalian cells. Mol Biol Evol 31:2194-211 (2014). PubMed: 24890374