RecombinantRabMAb

Recombinant Anti-TCTP antibody [EPR5540] (ab133568)

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Western blot - Anti-TCTP antibody [EPR5540] (ab133568)
  • Flow Cytometry - Anti-TCTP antibody [EPR5540] (ab133568)
  • Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (ab133568)
  • Anti-TCTP antibody [EPR5540] (ab133568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5540] to TCTP
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-TCTP antibody [EPR5540]
    See all TCTP primary antibodies

  • Description

    Rabbit monoclonal [EPR5540] to TCTP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region of Human TCTP (P13693).

  • Positive control

    • A431 cells; Raji, HeLa, Jurkat and A431 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5540

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (2)
1/10000 - 1/50000. Predicted molecular weight: 20 kDa.
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/250 - 1/500.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 20 kDa.
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/250 - 1/500.

Target

  • Function

    Involved in calcium binding and microtubule stabilization.

  • Tissue specificity

    Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.

  • Sequence similarities

    Belongs to the TCTP family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ27337 antibody
    • Fortilin antibody
    • Histamine releasing factor antibody
    • Histamine-releasing factor antibody
    • HRF antibody
    • Immunoglobulin E-dependent antibody
    • p02 antibody
    • p23 antibody
    • TCTP antibody
    • TCTP_HUMAN antibody
    • tpt1 antibody
    • Translationally controlled tumor protein antibody
    • Translationally-controlled tumor protein antibody
    • Tumor protein translationally controlled 1 antibody
    • tumor protein, translationally-controlled 1 antibody
    see all

Images

  • Western blot - Anti-TCTP antibody [EPR5540] (ab133568)
    Western blot - Anti-TCTP antibody [EPR5540] (ab133568)
    All lanes : Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa

  • Flow Cytometry - Anti-TCTP antibody [EPR5540] (ab133568)
    Flow Cytometry - Anti-TCTP antibody [EPR5540] (ab133568)
    Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (ab133568)
    Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (ab133568)
    Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.
  • Anti-TCTP antibody [EPR5540] (ab133568)
    Anti-TCTP antibody [EPR5540] (ab133568)

References (7)

Publishing research using ab133568? Please let us know so that we can cite the reference in this datasheet.

ab133568 has been referenced in 7 publications.

  • D'Amico S  et al. DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines. Cells 9:N/A (2020). PubMed: 32438775
  • Neuhäuser K  et al. Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling. Clin Transl Radiat Oncol 15:99-107 (2019). PubMed: 30815593
  • Sun R  et al. TCTP promotes epithelial-mesenchymal transition in lung adenocarcinoma. Onco Targets Ther 12:1641-1653 (2019). PubMed: 30881019
  • Sun Y  et al. miR-455-3p functions as a tumor suppressor in colorectal cancer and inhibits cell proliferation by targeting TPT1. Int J Clin Exp Pathol 11:2522-2529 (2018). PubMed: 31938365
  • Pinkaew D  et al. Fortilin binds IRE1a and prevents ER stress from signaling apoptotic cell death. Nat Commun 8:18 (2017). WB . PubMed: 28550308
  • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . PubMed: 25643152
  • Chen K  et al. Long-term artificial selection reveals a role of TCTP in autophagy in Mammalian cells. Mol Biol Evol 31:2194-211 (2014). PubMed: 24890374

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-TCTP antibody [EPR5540]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CT26(1,2,3,4) / MC38(5,6,7,8))
Gel Running Conditions
Reduced Non-Denaturing (Native) (15% SDS PAGE GEL)
Loading amount
15 µg
Specification
CT26(1,2,3,4) / MC38(5,6,7,8)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 24 2018

Western blot abreview for Anti-TCTP antibody [EPR5540]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (15%)
Sample
Mouse Cell lysate - whole cell (MIN 6 cell)
Specification
MIN 6 cell
Treatment
transfect TCTP plasmid into MIN 6 cell for 24 hours
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Jan 23 2014

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