• Product name
    Anti-TDG antibody [EPR8774]
    See all TDG primary antibodies
  • Description
    Rabbit monoclonal [EPR8774] to TDG
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TDG.
    Database link: Q13569

  • Positive control
    • Fetal lung tissue, Jurkat cell lysate, HeLa cells and HeLa cell lysate, K562 cell lysate and Fetal brain lysate
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab154192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
    • Sequence similarities
      Belongs to the TDG/mug DNA glycosylase family.
    • Post-translational
      Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • C JUN leucine zipper interactive protein antibody
      • C JUN leucine zipper interactive protein antibody
      • C-JUN leucine zipper interactive protein JZA-3 antibody
      • E130317C12Rik antibody
      • EC antibody
      • G/T mismatch specific thymine DNA glycosylase antibody
      • G/T mismatch specific thymine DNA glycosylase antibody
      • G/T mismatch-specific thymine DNA glycosylase antibody
      • JZA 3 antibody
      • Jza1 antibody
      • T:G mismatch thymine glycosylase antibody
      • Tdg antibody
      • TDG_HUMAN antibody
      • Thymine DNA glycosylase antibody
      • Thymine-DNA glycosylase antibody
      see all


    • All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/5000 dilution

      Lane 1 : Mouse brain tissue at 20 µg
      Lane 2 : mouse heart tissue at 20 µg
      Lane 3 : mouse kidney tissue
      Lane 4 : mouse spleen tissue at 20 µg
      Lane 5 : rat brain tissue at 20 µg
      Lane 6 : rat heart tissue at 20 µg
      Lane 7 : rat kidney tissue at 20 µg
      Lane 8 : rat spleen tissue at 20 µg

      Predicted band size: 46 kDa
      Observed band size: 55-60 kDa (why is the actual band size different from the predicted?)

      Exposure time: 3 minutes
    • All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/1000 dilution

      Lane 1 : Fetal lung cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : Fetal brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling TDG with ab154192 at 1/10 dilution (red) compared to a negative control rabbit IgG antibody (green).


    This product has been referenced in:
    • Neri F  et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics. Cell Rep N/A:N/A (2015). Read more (PubMed: 25660018) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (primary human fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Glycine)
    Loading amount
    40 µg
    control or anti-TDG siRNA
    primary human fibroblasts
    Blocking step
    Odyssey-Blocking buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

    Enni Markkanen

    Verified customer

    Submitted Feb 09 2016


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