Overview

  • Product name

    Anti-TDG antibody - N-terminal
    See all TDG primary antibodies
  • Description

    Rabbit polyclonal to TDG - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TDG (N terminal). The exact sequence is proprietary.
    Database link: Q13569

  • Positive control

    • WB: Jurkat, Raji and NCI-H929 whole cell lysates.
  • General notes

    Previously labelled as Thymine DNA glycosylase. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 46 kDa.

Target

  • Function

    In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
  • Sequence similarities

    Belongs to the TDG/mug DNA glycosylase family.
  • Post-translational
    modifications

    Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C JUN leucine zipper interactive protein antibody
    • C JUN leucine zipper interactive protein antibody
    • C-JUN leucine zipper interactive protein JZA-3 antibody
    • E130317C12Rik antibody
    • EC 3.2.2.29 antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch-specific thymine DNA glycosylase antibody
    • JZA 3 antibody
    • Jza1 antibody
    • T:G mismatch thymine glycosylase antibody
    • Tdg antibody
    • TDG_HUMAN antibody
    • Thymine DNA glycosylase antibody
    • Thymine-DNA glycosylase antibody
    see all

Images

  • All lanes : Anti-TDG antibody - N-terminal (ab227605) at 1/10000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10 % SDS-PAGE gel.

References

ab227605 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227605.
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