Key features and details
- Rabbit polyclonal to TDH
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-TDH antibody
DescriptionRabbit polyclonal to TDH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant full length protein corresponding to Human TDH aa 1-229. Two N-terminal tags, His-tag and GST-tag. Expressed in E.coli.
MLFIRMLRRAGQSPACGCWTPVLPVRFLGISPRQIPADANFHSASFSDTD HPRVLITGALGQLGVGLANLLRKRFGKDSVILSDIRKPPDHVFHSGPFIY SDILDYKNLREIVVNNRITWLFHYSALLSAFGEANVSLARAVNITGLHNI LDVAAEHNLQLFVPSTIGAFGPTSPRNPTPDLCIQRPRTIYGVSKVHAEL MGETMQSRFSMMPQSMANSSATWKPARDC
Database link: Q8IZJ6
- IHC-P: Human liver, cerebrum and glioma tissue. WB: Recombinant human TDH protein; Pig liver and kidney lysates; HepG2 cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevanceTDH (L threonine dehydrogenase) belongs to the sugar epimerase family and catalyzes the first of two steps in one of two L-threonine degradation pathways. According to reports, the human TDH gene is an expressed pseudogene encoding non-functional truncated proteins that are unable to make appropriate contacts with the substrates, L-threonine and NAD+.
- FLJ25033 antibody
- Inactive L threonine 3 dehydrogenase mitochondrial antibody
- L threonine dehydrogenase antibody
- SDR14E1P antibody
All lanes : Anti-TDH antibody (ab233402) at 1 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : Pig kidney tissue lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Anti-TDH antibody (ab233402) at 1 µg/ml + Recombinant human TDH protein.
Predicted band size: 25 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for TDH using ab233402 at 30µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for TDH using ab233402 at 20µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human glioma tissue stained for TDH using ab233402 at 20µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233402 has not yet been referenced specifically in any publications.