Key features and details
- Rabbit polyclonal to TDIF2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TDIF2 antibody
DescriptionRabbit polyclonal to TDIF2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: Control and probe#1 siRNA transfected U-2OS whole cell lyastes; HIHC-P: Human testis tissue; ICC: A431 whole cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
RelevanceDeoxynucleotidyltransferase terminal-interacting protein 2 (TDIF2)regulates the transcriptional activity of DNTT and ESR1 and may function as a chromatin remodeling protein.
- acidic 82 kDa protein mRNA antibody
- Deoxynucleotidyltransferase terminal-interacting protein 2 antibody
- DNTTIP2 antibody
All lanes : Anti-TDIF2 antibody (ab126481) at 0.4 µg/ml
Lane 1 : Target specific siRNA probe #1 transfected into U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : Target specific siRNA probe #2 transfected into U-2 OS whole cell lysate
Lane 3 : Control siRNA transfected into U-2 OS whole cell lysate
Predicted band size: 85 kDa
Immunohistochemical analysis of human testis tissue labeling TDIF2 in the nucleoli of subset cells in seminiferous ducts and Leydig cells with ab126481 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling TDIF2 in the nucleoli with ab126481 at 4 µg/ml.
ab126481 has not yet been referenced specifically in any publications.