Key features and details
- Rabbit polyclonal to TDP1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TDP1 antibody
See all TDP1 primary antibodies
DescriptionRabbit polyclonal to TDP1
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TDP1 aa 1-50. The exact sequence is proprietary. (NP_001008744.1).
Database link: Q9NUW8
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224822 was affinity purified using an epitope specific to TDP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 68 kDa.|
FunctionDNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
Tissue specificityUbiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
Involvement in diseaseSpinocerebellar ataxia, autosomal recessive, with axonal neuropathy
Sequence similaritiesBelongs to the tyrosyl-DNA phosphodiesterase family.
modificationsPhosphorylated on serine and/or threonine residues, but not on tyrosine residues.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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All lanes : Anti-TDP1 antibody (ab224822) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for TDP1 using ab224822 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
TDP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224822 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224822 at 0.4 µg/ml.
Lane 1: ab224822 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224822 has not yet been referenced specifically in any publications.