Anti-TDP1 antibody (ab227144)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TDP1 antibody
See all TDP1 primary antibodies -
Description
Rabbit polyclonal to TDP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment within Human TDP1 (internal sequence). The exact sequence is proprietary.
Database link: Q9NUW8 -
Positive control
- WB: HeLa, HEK-293T, A431 and HepG2 whole cell lysates. IHC-P: Human gastric tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 68 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. -
Tissue specificity
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. -
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy -
Sequence similarities
Belongs to the tyrosyl-DNA phosphodiesterase family. -
Post-translational
modificationsPhosphorylated on serine and/or threonine residues, but not on tyrosine residues. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55775 Human
- Omim: 607198 Human
- SwissProt: Q9NUW8 Human
- Unigene: 209945 Human
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Alternative names
- AI838772 antibody
- AW493413 antibody
- FLJ11090 antibody
see all
Images
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Western blot - Anti-TDP1 antibody (ab227144)All lanes : Anti-TDP1 antibody (ab227144) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (ab227144)Paraffin-embedded human gastric tissue stained for TDP1 using ab227144 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TDP1 antibody (ab227144)Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for TDP1 (green) using ab227144 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342.
References
ab227144 has not yet been referenced specifically in any publications.
