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    tdp1-antibody-ab227144.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Topoisomerases
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Anti-TDP1 antibody (ab227144)

  • Datasheet
  • SDS
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Western blot - Anti-TDP1 antibody (ab227144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (ab227144)
  • Immunocytochemistry/ Immunofluorescence - Anti-TDP1 antibody (ab227144)

Key features and details

  • Rabbit polyclonal to TDP1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human TDP1 protein (ab131921)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TDP1 antibody
    See all TDP1 primary antibodies
  • Description

    Rabbit polyclonal to TDP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human TDP1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NUW8

  • Positive control

    • WB: HeLa, HEK-293T, A431 and HepG2 whole cell lysates. IHC-P: Human gastric tissue. ICC/IF: A431 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human TDP1 protein (ab131921)
  • Related Products

    • Recombinant Human TDP1 protein (ab131921)

Applications

Our Abpromise guarantee covers the use of ab227144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
  • Tissue specificity

    Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
  • Involvement in disease

    Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy
  • Sequence similarities

    Belongs to the tyrosyl-DNA phosphodiesterase family.
  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession Q9NUW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55775 Human
    • Omim: 607198 Human
    • SwissProt: Q9NUW8 Human
    • Unigene: 209945 Human
    • Alternative names

      • AI838772 antibody
      • AW493413 antibody
      • FLJ11090 antibody
      • MGC104252 antibody
      • MGC112732 antibody
      • RP24-311F12.2 antibody
      • SCAN1 antibody
      • TDP1 antibody
      • TYDP antibody
      • TYDP1_HUMAN antibody
      • Tyr-DNA phosphodiesterase 1 antibody
      • Tyrosyl-DNA phosphodiesterase 1 antibody
      see all

    Images

    • Western blot - Anti-TDP1 antibody (ab227144)
      Western blot - Anti-TDP1 antibody (ab227144)
      All lanes : Anti-TDP1 antibody (ab227144) at 1/5000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 68 kDa



      7.5% SDS-PAGE gel.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (ab227144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (ab227144)

      Paraffin-embedded human gastric tissue stained for TDP1 using ab227144 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-TDP1 antibody (ab227144)
      Immunocytochemistry/ Immunofluorescence - Anti-TDP1 antibody (ab227144)

      Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for TDP1 (green) using ab227144 at 1/200 dilution in ICC/IF.

      Nuclear counterstain: Hoechst 33342.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227144? Please let us know so that we can cite the reference in this datasheet.

    ab227144 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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