Anti-TDP1 antibody (ab4166)
Key features and details
- Rabbit polyclonal to TDP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-TDP1 antibody
See all TDP1 primary antibodies -
Description
Rabbit polyclonal to TDP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
Abcam recommends blocking with 1-3% Milk. |
Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa). Abcam recommends blocking with 1-3% Milk. |
Target
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Function
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. -
Tissue specificity
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. -
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy -
Sequence similarities
Belongs to the tyrosyl-DNA phosphodiesterase family. -
Post-translational
modificationsPhosphorylated on serine and/or threonine residues, but not on tyrosine residues. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55775 Human
- Entrez Gene: 104884 Mouse
- Entrez Gene: 314380 Rat
- Omim: 607198 Human
- SwissProt: Q9NUW8 Human
- SwissProt: Q8BJ37 Mouse
- SwissProt: Q4G056 Rat
- Unigene: 209945 Human
see all -
Alternative names
- AI838772 antibody
- AW493413 antibody
- FLJ11090 antibody
see all
Images
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Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Lane 1 : Molecular weight markers
Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution
Lane 2 : 1 ng purified recombinant human TDP1
Lane 3 : 3 ng purified recombinant human TDP1
Lane 4 : 6 ng purified recombinant human TDP1
Lane 5 : 6 micrograms total protein from MCF7 human cell extract
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaab4166 was incubated for 1 hr at room temp.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab4166 has been referenced in 26 publications.
- Meroni A et al. NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors. Sci Adv 8:eabq0648 (2022). PubMed: 36490343
- Leung E et al. Validating TDP1 as an Inhibition Target for the Development of Chemosensitizers for Camptothecin-Based Chemotherapy Drugs. Oncol Ther 9:541-556 (2021). PubMed: 34159519
- Álvarez-Quilón A et al. Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease. Mol Cell 78:1152-1165.e8 (2020). PubMed: 32516598
- Saha S et al. DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks. Cell Rep 33:108569 (2020). PubMed: 33378676
- Ghosh A et al. SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy. Sci Adv 5:eaax9778 (2019). PubMed: 31723605