Western blot abreview for Anti-TDP43 antibody [2E2-D3]

Poor
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (myoblase and C2C12)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
3 µg
Specification
myoblase and C2C12
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk

Secondary antibody

Name
Non-Abcam antibody was used: jackson labs hrp anti mouse secondary
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 45 weak kDa Non-specific: 38, 56 kDa

Additional data

Abcam response
This Abreview was produced as a part of our Abtrial program.

Dr. wei xia

Verified customer

Submitted Feb 25 2019

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