Overview

  • Product name

  • Description

    Rabbit polyclonal to TDP43
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human TDP43 aa 150-200. The exact sequence is proprietary. NP_031401.1
    Database link: Q13148

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human non-small cell lung cancer tissue; mouse renal cell carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab225710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.

Target

  • Function

    DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
  • Tissue specificity

    Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
  • Involvement in disease

    Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The RRM domains can bind to both DNA and RNA.
  • Post-translational
    modifications

    Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  • Cellular localization

    Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALS10 antibody
    • OTTHUMP00000002171 antibody
    • OTTHUMP00000002172 antibody
    • OTTHUMP00000002173 antibody
    • TADBP_HUMAN antibody
    • TAR DNA binding protein 43 antibody
    • TAR DNA binding protein antibody
    • TAR DNA-binding protein 43 antibody
    • TARDBP antibody
    • TDP 43 antibody
    • TDP-43 antibody
    • TDP43 antibody
    see all

Images

  • Formalin fixed, paraffin embedded mouse renal cell carcinoma tissue stained for TDP43 with ab225710 (1/200 dilution) in immunohistochemical analysis. DAB detection.

  • All lanes : Anti-TDP43 antibody (ab225710) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg/ml
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg/ml
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg/ml
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg/ml
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg/ml

    Developed using the ECL technique.

    Predicted band size: 45 kDa


    Exposure time: 30 seconds
  • Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225710 used for for WB at 0.4 µg/mg and IP at 6 µg/mg lysate.

    Lane 1: ab225710 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence 10 seconds.

  • Formalin fixed, paraffin embedded human non-small cell lung cancer tissue stained for TDP43 with ab225710 (1/1000 dilution) in immunohistochemical analysis. DAB detection.

References

ab225710 has not yet been referenced specifically in any publications.

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