Anti-TDP43 antibody (ab80608)
Key features and details
- Goat polyclonal to TDP43
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TDP43 antibody
See all TDP43 primary antibodies -
Description
Goat polyclonal to TDP43 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human TDP43 aa 256-269 (internal sequence) (Cysteine residue).
Sequence:C-HISNAEPKHNSNRQ
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Positive control
- Human lysates of cell line Jurkat
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab80608 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
Chicken
Cow
Dog
Pig
Zebrafish
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 45 kDa.
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 45 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. -
Tissue specificity
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. -
Involvement in disease
Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The RRM domains can bind to both DNA and RNA. -
Post-translational
modificationsHyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. -
Cellular localization
Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies. - Information by UniProt
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Database links
- Entrez Gene: 419453 Chicken
- Entrez Gene: 23435 Human
- Entrez Gene: 230908 Mouse
- Entrez Gene: 298648 Rat
- Entrez Gene: 325052 Zebrafish
- Omim: 605078 Human
- SwissProt: Q5ZLN5 Chicken
- SwissProt: Q13148 Human
see all -
Alternative names
- ALS10 antibody
- OTTHUMP00000002171 antibody
- OTTHUMP00000002172 antibody
see all
Images
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Western blot - Anti-TDP43 antibody (ab80608)All lanes : Anti-TDP43 antibody (ab80608) at 1 µg/ml
Lane 1 : Human cerebellum cell lysate
Lane 2 : Human frontal cotex cell lysate
Lane 3 : Human hippocampus cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDa -
Western blot - Anti-TDP43 antibody (ab80608)Anti-TDP43 antibody (ab80608) at 0.3 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab80608 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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