Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Dorsal Root Ganglion)
Specification
Dorsal Root Ganglion
Other product details
Incubation time
72 hour(s) and 0 minute(s)
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Fab Fragment Donkey Anti-Rabbit Ig
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
DRG tissue was fixed in 4% PFA 24Hr at Room Temperature, followed by cryoprotection and cutting on cryostat (60um) for free floating processing. TDP43 labelled neuronal nuclei and satellite glial nuclei, as expected, very brightly and with minimal background. Scale bar in image: 50um.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Steven West
Verified customer
Submitted Oct 27 2017