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    tdrd7-antibody-ab241349.pdf

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Anti-TDRD7 antibody (ab241349)

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Western blot - Anti-TDRD7 antibody (ab241349)

    Key features and details

    • Rabbit polyclonal to TDRD7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-TDRD7 antibody
      See all TDRD7 primary antibodies
    • Description

      Rabbit polyclonal to TDRD7
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Orangutan
    • Immunogen

      Synthetic peptide within Human TDRD7 aa 1048-1098. The exact sequence is proprietary.
      Database link: Q8NHU6

    • Positive control

      • WB: HeLa, Jurkat and HEK-293T whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibody was affinity purified using an epitope specific to TDRD7 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • RNAi
      • Epigenetics and Nuclear Signaling
      • RNAi
      • Argonaute and Piwi

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)

    Applications

    Our Abpromise guarantee covers the use of ab241349 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/5000.

    Target

    • Sequence similarities

      Contains 2 Tudor domains.
    • Cellular localization

      Cytoplasm. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids.
    • Target information above from: UniProt accession Q8NHU6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23424 Human
      • Entrez Gene: 100174458 Orangutan
      • Omim: 611258 Human
      • SwissProt: Q8NHU6 Human
      • SwissProt: Q5RAH6 Orangutan
      • Unigene: 193842 Human
      • Form

        Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.
      • Alternative names

        • CATC4 antibody
        • KIAA1529 antibody
        • PCTAIRE 2 antibody
        • PCTAIRE2 binding protein antibody
        • PCTAIRE2 BP antibody
        • PCTAIRE2-binding protein antibody
        • PCTAIRE2BP antibody
        • RP11-508D10.1 antibody
        • TDRD 7 antibody
        • Tdrd7 antibody
        • TDRD7_HUMAN antibody
        • TrAP antibody
        • Tudor domain containing 7 antibody
        • Tudor domain-containing protein 7 antibody
        • Tudor repeat associator with PCTAIRE 2 antibody
        see all

      Images

      • Western blot - Anti-TDRD7 antibody (ab241349)
        Western blot - Anti-TDRD7 antibody (ab241349)
        All lanes : Anti-TDRD7 antibody (ab241349) at 0.4 µg/ml

        Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
        Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
        Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

        Lysates/proteins at 50 µg per lane.

        Exposure time: 30 seconds


        Prepared using NETN lysis buffer.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab241349? Please let us know so that we can cite the reference in this datasheet.

      ab241349 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab241349.
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