Overview

  • Product name

    Anti-TDRD9 antibody
  • Description

    Rabbit polyclonal to TDRD9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human TDRD9 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab135462)

  • Positive control

    • WB: Mouse and rat testis tissue lysate. IHC-P: Human normal testis tissue. ICC/IF: Hek293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 155 kDa). Abcam recommends using milk as the blocking agent (3%).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 Tudor domain.
  • Cellular localization

    Cytoplasm. Nucleus. Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf75 antibody
    • chromosome 14 open reading frame 75 antibody
    • DKFZp434N0820 antibody
    • FLJ36164 antibody
    • HIG 1 antibody
    • Hypoxia inducible HIG 1 antibody
    • MGC135025 antibody
    • NET54 antibody
    • Putative ATP dependent RNA helicase TDRD9 antibody
    • Putative ATP-dependent RNA helicase TDRD9 antibody
    • TDRD 9 antibody
    • Tdrd9 antibody
    • TDRD9_HUMAN antibody
    • Tudor domain containing 9 antibody
    • Tudor domain containing protein 9 antibody
    • Tudor domain-containing protein 9 antibody
    see all

Images

  • Anti-TDRD9 antibody (ab118427) at 1 µg/ml (Milk blocking - 3%) + Testis (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 155 kDa
    Observed band size: 171 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 190 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • IHC image of TDRD9 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab118427, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TDRD9 antibody (ab118427) at 1 µg/ml

    Lane 1 : MCF7 cells (human).
    Lane 2 : MDA231 cells (human).

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 155 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • ICC/IF image of ab118427 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118427, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% paraformaldehyde fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • All lanes : Anti-TDRD9 antibody (ab118427) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Testis (Rat) Tissue Lysate
    Lane 2 : Testis (Rat) Tissue Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 155 kDa
    Observed band size: 171 kDa why is the actual band size different from the predicted?
    Additional bands at: 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Abcam recommends using milk as the blocking agent (3%).

References

This product has been referenced in:

  • Murat P  et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). Read more (PubMed: 30591072) »
See 1 Publication for this product

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