Overview

  • Product name

    Anti-TdT antibody - C-terminal
    See all TdT primary antibodies
  • Description

    Rabbit polyclonal to TdT - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human TdT (C terminal). The exact sequence is proprietary.
    Database link: P04053

  • Positive control

    • WB: Jurkat whole cell and nuclear extracts; TdT transfected HEK-293T whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 59 kDa.

Target

  • Function

    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
  • Sequence similarities

    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deoxynucleotidyltransferase terminal antibody
    • DNA nucleotidylexotransferase antibody
    • DNTT antibody
    • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
    • TDT antibody
    • TDT_HUMAN antibody
    • Terminal addition enzyme antibody
    • Terminal deoxynucleotidyltransferase antibody
    • Terminal deoxyribonucleotidyltransferase antibody
    • Terminal transferase antibody
    see all

Images

  • All lanes : Anti-TdT antibody - C-terminal (ab226953) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 59 kDa



    7.5 % SDS-PAGE gel.

  • All lanes : Anti-TdT antibody - C-terminal (ab226953) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : TdT transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 59 kDa



    7.5 % SDS-PAGE gel.

References

ab226953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226953.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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