Recombinant
RabMAb

Recombinant Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)

Overview

  • Product name

    Anti-TdT antibody [EPR2976Y] - BSA and Azide free
    See all TdT primary antibodies
  • Description

    Rabbit monoclonal [EPR2976Y] to TdT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TdT aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P04053

  • Positive control

    • ICC/IF: MOLT-4 cells.
  • General notes

    Ab232378 is the carrier-free version of ab76544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2976Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
  • Sequence similarities

    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deoxynucleotidyltransferase terminal antibody
    • DNA nucleotidylexotransferase antibody
    • DNTT antibody
    • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
    • TDT antibody
    • TDT_HUMAN antibody
    • Terminal addition enzyme antibody
    • Terminal deoxynucleotidyltransferase antibody
    • Terminal deoxyribonucleotidyltransferase antibody
    • Terminal transferase antibody
    see all

Images

  • ab76544 (purified) at 1:20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

    Lane 1 (input): Jurkat (human acute T cell leukemia) whole cell lysate 10μg
    Lane 2 (+): ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

  • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

  • Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1:100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

References

ab232378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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