Recombinant
RabMAb

Recombinant Anti-TdT antibody [EPR9732] (ab155082)

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Overview

  • Product name

    Anti-TdT antibody [EPR9732]
    See all TdT primary antibodies

  • Description

    Rabbit monoclonal [EPR9732] to TdT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human TdT.

  • Positive control

    • Human fetal thymus and Jurkat cell lysates; Human thymoma and thymus tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9732

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

    • Sequence similarities

      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Deoxynucleotidyltransferase terminal antibody
        • DNA nucleotidylexotransferase antibody
        • DNTT antibody
        • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
        • TDT antibody
        • TDT_HUMAN antibody
        • Terminal addition enzyme antibody
        • Terminal deoxynucleotidyltransferase antibody
        • Terminal deoxyribonucleotidyltransferase antibody
        • Terminal transferase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)

        Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling TdT with ab155082 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)

        Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling TdT with ab155082 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-TdT antibody [EPR9732] (ab155082)
        Western blot - Anti-TdT antibody [EPR9732] (ab155082)
        All lanes : Anti-TdT antibody [EPR9732] (ab155082) at 1/1000 dilution

        Lane 1 : Human fetal thymus cell lysate
        Lane 2 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 59 kDa

      References

      This product has been referenced in:

      • Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). Read more (PubMed: 25775545) »
      See 1 Publication for this product

      Publishing research using ab155082? Please let us know so that we can cite the reference in this datasheet.

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