
Anti-TdT antibody [EPR9732] (ab155082)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-TdT antibody [EPR9732]
See all TdT primary antibodies -
DescriptionRabbit monoclonal [EPR9732] to TdT
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to residues in Human TdT (UniProt: P04053).
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Positive control
- Human fetal thymus and Jurkat cell lysates; Human thymoma and thymus tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading...
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PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR9732
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 59 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionTemplate-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
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Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1791 Human
- Omim: 187410 Human
- SwissProt: P04053 Human
- Unigene: 534206 Human
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Alternative names
- Deoxynucleotidyltransferase terminal antibody
- DNA nucleotidylexotransferase antibody
- DNTT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling TdT with ab155082 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling TdT with ab155082 at 1/100 dilution.
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All lanes : Anti-TdT antibody [EPR9732] (ab155082) at 1/1000 dilution
Lane 1 : Human fetal thymus cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 59 kDa
Datasheets and documents
References
This product has been referenced in:
- Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). Read more (PubMed: 25775545) »