Anti-TdT antibody [EPR9732] Recombinant (ab155082)

Product name

Anti-TdT antibody [EPR9732]
See all TdT primary antibodies
Description

Rabbit monoclonal [EPR9732] to TdT
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment, corresponding to residues in Human TdT (UniProt: P04053).
Positive control
- Human fetal thymus and Jurkat cell lysates; Human thymoma and thymus tissues.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9732
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab155082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Sequence similarities

Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P04053 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1791 Human
- Omim: 187410 Human
- SwissProt: P04053 Human
- Unigene: 534206 Human
Alternative names
- Deoxynucleotidyltransferase terminal antibody
- DNA nucleotidylexotransferase antibody
- DNTT antibody
- Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
- TDT antibody
- TDT_HUMAN antibody
- Terminal addition enzyme antibody
- Terminal deoxynucleotidyltransferase antibody
- Terminal deoxyribonucleotidyltransferase antibody
- Terminal transferase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)

Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling TdT with ab155082 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling TdT with ab155082 at 1/100 dilution.
Western blot - Anti-TdT antibody [EPR9732] (ab155082)

All lanes : Anti-TdT antibody [EPR9732] (ab155082) at 1/1000 dilution

Lane 1 : Human fetal thymus cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 59 kDa

Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). Read more (PubMed: 25775545) »

Publishing research using ab155082? Please let us know so that we can cite the reference in this datasheet.

Anti-TdT antibody [EPR9732] (ab155082)