Overview

  • Product name
    Anti-TdT antibody [EPR9732]
    See all TdT primary antibodies
  • Description
    Rabbit monoclonal [EPR9732] to TdT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to residues in Human TdT (UniProt: P04053).

  • Positive control
    • Human fetal thymus and Jurkat cell lysates; Human thymoma and thymus tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR9732
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab155082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function
      Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
    • Sequence similarities
      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      • Alternative names
        • Deoxynucleotidyltransferase terminal antibody
        • DNA nucleotidylexotransferase antibody
        • DNTT antibody
        • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
        • TDT antibody
        • TDT_HUMAN antibody
        • Terminal addition enzyme antibody
        • Terminal deoxynucleotidyltransferase antibody
        • Terminal deoxyribonucleotidyltransferase antibody
        • Terminal transferase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
        Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling TdT with ab155082 at 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
        Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling TdT with ab155082 at 1/100 dilution.
      • Western blot - Anti-TdT antibody [EPR9732] (ab155082)
        Western blot - Anti-TdT antibody [EPR9732] (ab155082)
        All lanes : Anti-TdT antibody [EPR9732] (ab155082) at 1/1000 dilution

        Lane 1 : Human fetal thymus cell lysate
        Lane 2 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 59 kDa

      References

      This product has been referenced in:
      • Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). Read more (PubMed: 25775545) »

      See 1 Publication for this product

      Publishing research using ab155082? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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