Recombinant Anti-TdT antibody [SP150] (ab183341)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP150] to TdT
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TdT antibody [SP150]
See all TdT primary antibodies -
Description
Rabbit monoclonal [SP150] to TdT -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human thymus and thymoma tissue. WB: Jurkat whole cell lysate (ab7899). Flow Cyt (Intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP150 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
|
WB |
1/100. Predicted molecular weight: 59 kDa.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
WB
1/100. Predicted molecular weight: 59 kDa. |
Target
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Function
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. -
Sequence similarities
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1791 Human
- Omim: 187410 Human
- SwissProt: P04053 Human
- Unigene: 534206 Human
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Alternative names
- Deoxynucleotidyltransferase terminal antibody
- DNA nucleotidylexotransferase antibody
- DNTT antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling TdT with ab183341 at a 1/100 dilution.
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Anti-TdT antibody [SP150] (ab183341) at 1/100 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 59 kDa -
Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling TdT with ab183341 (green), compared with a negative control of rabbit IgG (blue).
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Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling TdT with ab183341 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183341 has not yet been referenced specifically in any publications.