Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP150] to TdT
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
Product nameAnti-TdT antibody [SP150]
See all TdT primary antibodies
DescriptionRabbit monoclonal [SP150] to TdT
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TdT aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P04053
- IHC-P: Human thymus and thymoma tissue. WB: Jurkat whole cell lysate (ab7899). Flow Cyt: Jurkat cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab183341 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
|WB||1/100. Predicted molecular weight: 59 kDa.|
FunctionTemplate-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
- Information by UniProt
- Deoxynucleotidyltransferase terminal antibody
- DNA nucleotidylexotransferase antibody
- DNTT antibody
Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling TdT with ab183341 at a 1/100 dilution.
Anti-TdT antibody [SP150] (ab183341) at 1/100 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 59 kDa
Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling TdT with ab183341 (green), compared with a negative control of rabbit IgG (blue).
Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling TdT with ab183341 at a 1/100 dilution.
ab183341 has not yet been referenced specifically in any publications.