Recombinant
RabMAb

Anti-TdT antibody [SP150] (ab183341)

Overview

  • Product name
    Anti-TdT antibody [SP150]
    See all TdT primary antibodies
  • Description
    Rabbit monoclonal [SP150] to TdT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TdT aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: P04053

  • Positive control
    • Jurkat cell lysate, Human thymus and thymoma tissues, Jurkat cells.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purification notes
    Purified from TCS by protein A/G.
  • Clonality
    Monoclonal
  • Clone number
    SP150
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab183341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

WB 1/100. Predicted molecular weight: 59 kDa.

Target

  • Function
    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
  • Sequence similarities
    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deoxynucleotidyltransferase terminal antibody
    • DNA nucleotidylexotransferase antibody
    • DNTT antibody
    • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
    • TDT antibody
    • TDT_HUMAN antibody
    • Terminal addition enzyme antibody
    • Terminal deoxynucleotidyltransferase antibody
    • Terminal deoxyribonucleotidyltransferase antibody
    • Terminal transferase antibody
    see all

Images

  • Anti-TdT antibody [SP150] (ab183341) at 1/100 dilution + Jurkat cell lysate

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded Human thymus tissue, labeling TdT with ab183341 at 1/100 dilution.

  • Flow cytometric analysis of Jurkat cells, labeling TdT with ab183341 (green), compared with a negative control of rabbit IgG (blue).

  • Immunohistochemical analysis of paraffin-embedded Human thymoma tissue, labeling TdT with ab183341 at 1/100 dilution.

References

ab183341 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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