Overview

  • Product name

    Anti-TEA domain family member 2/ETF antibody [404C5a]
    See all TEA domain family member 2/ETF primary antibodies
  • Description

    Mouse monoclonal [404C5a] to TEA domain family member 2/ETF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TEA domain family member 2/ETF.

  • General notes

    Previously labelled as TEA domain family member 2. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    404C5a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
  • Sequence similarities

    Contains 1 TEA DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Embryonic TEA domain containing factor antibody
    • Etdf antibody
    • ETEF 1 antibody
    • ETF antibody
    • TEA domain family member 2 antibody
    • TEAD 2 antibody
    • TEAD-2 antibody
    • TEAD2 antibody
    • Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody
    • TEAD2_HUMAN antibody
    • TEF 4 antibody
    • TEF4 antibody
    • Transcriptional enhancer factor 4 antibody
    • Transcriptional enhancer factor TEF 4 antibody
    • Transcriptional enhancer factor TEF-4 antibody
    see all

Images

  • Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374) + immunizing recombinant protein

    Predicted band size: 49 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.

References

ab54374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 23 2014

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