Key features and details
- Mouse monoclonal [404C5a] to TEA domain family member 2/ETF
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-TEA domain family member 2/ETF antibody [404C5a]
See all TEA domain family member 2/ETF primary antibodies
DescriptionMouse monoclonal [404C5a] to TEA domain family member 2/ETF
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TEA domain family member 2/ETF.
Previously labelled as TEA domain family member 2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab54374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
Sequence similaritiesContains 1 TEA DNA-binding domain.
- Information by UniProt
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374) + immunizing recombinant protein
Predicted band size: 49 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.
ab54374 has not yet been referenced specifically in any publications.