Key features and details
- Rabbit polyclonal to TEA domain family member 2/ETF
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TEA domain family member 2/ETF antibody
See all TEA domain family member 2/ETF primary antibodies
DescriptionRabbit polyclonal to TEA domain family member 2/ETF
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human TEA domain family member 2/ETF (internal sequence).
Database link: Q15562
- Human breast carcinoma tissue; HepG2 cells.
Previously labelled as TEA domain family member 2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab196669 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab196669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/500.|
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
Sequence similaritiesContains 1 TEA DNA-binding domain.
- Information by UniProt
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TEA domain family member 2/ETF with ab196669 at 1/50 dilution (left) and synthesized peptide (right).
Immunofluorescent analysis of HepG2 cells labeling TEA domain family member 2/ETF with ab196669 at 1/100 dilution (left) and synthesized peptide (right).
ab196669 has been referenced in 1 publication.
- Tome-Garcia J et al. Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma. Nat Commun 9:4020 (2018). PubMed: 30275445