Overview

  • Product name

    Anti-TEA domain family member 2/ETF antibody
    See all TEA domain family member 2/ETF primary antibodies
  • Description

    Rabbit polyclonal to TEA domain family member 2/ETF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human TEA domain family member 2/ETF aa 2-51 (N terminal).
    Sequence:

    GEPRAGAALDDGSGWTGSEEGSEEGTGGSEGAGGDGGPDAEGVWSPDIEQ


    Database link: NP_003589

  • Positive control

    • Fetal brain lysate.
  • General notes

    Previously labelled as TEA domain family member 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab83670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/12500.

Target

  • Function

    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
  • Sequence similarities

    Contains 1 TEA DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Embryonic TEA domain containing factor antibody
    • Etdf antibody
    • ETEF 1 antibody
    • ETF antibody
    • TEA domain family member 2 antibody
    • TEAD 2 antibody
    • TEAD-2 antibody
    • TEAD2 antibody
    • Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody
    • TEAD2_HUMAN antibody
    • TEF 4 antibody
    • TEF4 antibody
    • Transcriptional enhancer factor 4 antibody
    • Transcriptional enhancer factor TEF 4 antibody
    • Transcriptional enhancer factor TEF-4 antibody
    see all

Images

  • Anti-TEA domain family member 2/ETF antibody (ab83670) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Gel concentration 12%

References

This product has been referenced in:

  • Lu J  et al. IKBKE regulates cell proliferation and epithelial-mesenchymal transition of human malignant glioma via the Hippo pathway. Oncotarget 8:49502-49514 (2017). Read more (PubMed: 28548934) »
See 1 Publication for this product

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