Anti-TEA domain family member 2/ETF antibody (ab92279)
Key features and details
- Goat polyclonal to TEA domain family member 2/ETF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TEA domain family member 2/ETF antibody
See all TEA domain family member 2/ETF primary antibodies -
Description
Goat polyclonal to TEA domain family member 2/ETF -
Host species
Goat -
Specificity
Not expected to cross-react with other TEA domain family proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide corresponding to Human TEA domain family member 2/ETF aa 238-249 (internal sequence).
Sequence:C-EPPDAVDSYQRH
Database link: NP_003589.1 -
Positive control
- Human ileum tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. -
Sequence similarities
Contains 1 TEA DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8463 Human
- Entrez Gene: 21677 Mouse
- Entrez Gene: 308582 Rat
- Omim: 601729 Human
- SwissProt: Q15562 Human
- SwissProt: P48301 Mouse
- Unigene: 515534 Human
- Unigene: 3019 Mouse
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Alternative names
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
see all
Images
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Anti-TEA domain family member 2/ETF antibody (ab92279) at 1 µg/ml + Human colon lysate (35µg protein in RIPA buffer)
Predicted band size: 49 kDaDetected by chemiluminescence.
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Anti-TEA domain family member 2/ETF antibody (ab92279) at 1 µg/ml + Human ileum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (4)
ab92279 has been referenced in 4 publications.
- Holmes B et al. mTORC2-mediated direct phosphorylation regulates YAP activity promoting glioblastoma growth and invasive characteristics. Neoplasia 23:951-965 (2021). PubMed: 34343821
- Chang Y et al. PHF5A promotes colorectal cancerprogression by alternative splicing of TEAD2. Mol Ther Nucleic Acids 26:1215-1227 (2021). PubMed: 34853721
- Liu G et al. Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis. Aging (Albany NY) 12:11530-11549 (2020). PubMed: 32541093
- Wang J et al. Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells. Am J Transl Res 10:998-1011 (2018). WB ; Cow . PubMed: 29636889