Key features and details
- Goat polyclonal to TEA domain family member 2/ETF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TEA domain family member 2/ETF antibody
See all TEA domain family member 2/ETF primary antibodies
DescriptionGoat polyclonal to TEA domain family member 2/ETF
SpecificityNot expected to cross-react with other TEA domain family proteins.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- Human ileum tissue lysate
Previously labelled as TEA domain family member 2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab92279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product.
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
Sequence similaritiesContains 1 TEA DNA-binding domain.
- Information by UniProt
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
Anti-TEA domain family member 2/ETF antibody (ab92279) at 1 µg/ml + Human colon lysate (35µg protein in RIPA buffer)
Predicted band size: 49 kDa
Detected by chemiluminescence.
Anti-TEA domain family member 2/ETF antibody (ab92279) at 1 µg/ml + Human ileum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab92279 has been referenced in 1 publication.
- Wang J et al. Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells. Am J Transl Res 10:998-1011 (2018). WB ; Cow . PubMed: 29636889