Overview

  • Product name

  • Description

    Rabbit polyclonal to TEAD4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human TEAD4 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab151396)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab151274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
  • Tissue specificity

    Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
  • Sequence similarities

    Contains 1 TEA DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EFTR 2 antibody
    • EFTR2 antibody
    • hRTEF 1B antibody
    • hRTEF1B antibody
    • MGC9014 antibody
    • OTTHUMP00000238119 antibody
    • OTTHUMP00000238122 antibody
    • OTTHUMP00000238124 antibody
    • Related to TEF 1 antibody
    • Related to TEF1 antibody
    • Related transcription enhancer factor 1B antibody
    • RTEF1 antibody
    • TCF13L1 antibody
    • TEA domain family member 4 antibody
    • TEAD 4 antibody
    • TEAD-4 antibody
    • TEAD4 antibody
    • TEAD4_HUMAN antibody
    • TEF 3 antibody
    • TEF3 antibody
    • TEFR 1 antibody
    • TEFR1 antibody
    • Transcription factor 13 (SV40 transcriptional enhancer factor) like 1 antibody
    • Transcription factor 13 like 1 antibody
    • Transcription factor 13-like 1 antibody
    • Transcription factor RTEF 1 antibody
    • Transcription factor RTEF-1 antibody
    • Transcription factor RTEF1 antibody
    • Transcriptional enhancer factor 1 related antibody
    • Transcriptional enhancer factor 3 antibody
    • Transcriptional enhancer factor TEF 3 antibody
    • Transcriptional enhancer factor TEF-3 antibody
    • Transcriptional enhancer factor TEF3 antibody
    see all

Images

  • All lanes : Anti-TEAD4 antibody (ab151274) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TEAD4 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 48 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab151274 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab151274 was shown to recognize TEAD4 in wild-type HAP1 cells as signal was lost at the expected MW in TEAD4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TEAD4 knockout samples were subjected to SDS-PAGE. Ab151274 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-TEAD4 antibody (ab151274) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 32 kDa, 40 kDa, 66 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151274 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab151274 has not yet been referenced specifically in any publications.

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