Product nameAnti-TEAD4 antibody
See all TEAD4 primary antibodies
DescriptionRabbit polyclonal to TEAD4
SpecificityThis antibody reacts with TEAD4
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
- 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab50945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
Tissue specificityPreferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Sequence similaritiesContains 1 TEA DNA-binding domain.
- Information by UniProt
- EFTR 2 antibody
- EFTR2 antibody
- hRTEF 1B antibody
Lane 2 : Anti-TEAD4 antibody (ab50945) at 0.25 µg/ml
Lane 1 : MARKER
Lane 2 : 293T cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemistry of Human Pancreas lysate tissue at an antibody concentration of 5.0µg/ml using anti-TEAD4 antibody (ab50945)
ab50945 has not yet been referenced specifically in any publications.