Product nameAnti-TEAD4 antibody
See all TEAD4 primary antibodies
DescriptionRabbit polyclonal to TEAD4
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Cow, Human
Predicted to work with: Mouse, Pig
Recombinant protein fragment corresponding to a region within amino acids 1 - 230 of Human TEAD4 (NP_003204).
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IHC-P: SW480 xenograft IF: HepG2 cell line
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab97460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
Tissue specificityPreferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Sequence similaritiesContains 1 TEA DNA-binding domain.
- Information by UniProt
- EFTR 2 antibody
- EFTR2 antibody
- hRTEF 1B antibody
Anti-TEAD4 antibody (ab97460) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
ab97460, at 1/500 dilution, staining TEAD4 in a paraffin-embedded SW480 (human) xenograft by Immunohistochemical analysis
ICC/IF image of ab97460 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Hwang JH et al. TAZ couples Hippo/Wnt signalling and insulin sensitivity through Irs1 expression. Nat Commun 10:421 (2019). Read more (PubMed: 30679431) »
- Ma R et al. Activated YAP causes renal damage of type 2 diabetic nephropathy. Eur Rev Med Pharmacol Sci 23:755-763 (2019). Read more (PubMed: 30720184) »